They are potted into porous, sterile substrates (perlite, vermiculite, or peat moss).
Preserving endangered plant species. 5. Finding High-Quality PPTs and PDFs
They are kept under high humidity and lower light intensities, which are gradually decreased over several weeks to "harden" them for the harsh outdoor environment. 4. Components of the Culture Medium
The established explant is transferred to a medium containing specific hormones (usually cytokinins) to encourage the growth of multiple shoots. plant tissue culture ppt pdf
Promotes callus (unorganized tissue) formation. E. Incubation and Growth
The clean zone where explants are inoculated. It relies heavily on Laminar Air Flow (LAF) cabinets , which use High-Efficiency Particulate Air (HEPA) filters to remove 99.97% of airborne particles and spores.
: Endangered species can be propagated and preserved without depleting wild populations. 3. Basic Components of a Culture Medium They are potted into porous, sterile substrates (perlite,
: Nitrogen (N), Phosphorus (P), Potassium (K), Calcium (Ca), Magnesium (Mg), and Sulfur (S) required in higher quantities.
specifically covers essential lab operations like autoclaving, filter sterilization, and surface disinfection. Application Overview
Usually 2–3% sucrose, as in vitro plants have limited photosynthetic capacity. Finding High-Quality PPTs and PDFs They are kept
Utilizing plant cell factories to harvest therapeutic compounds (e.g., Paclitaxel/Taxol for cancer treatment, Shikonin, Ginsenosides) without destroying natural forests.
Used at 10–20% concentration for 10–20 minutes. Mercuric Chloride ( HgCl2HgCl sub 2